Journal: Polymers

Article Title: Mechanical and Thermal Behaviours of Weft-Knitted Spacer Fabric Structure with Inlays for Insole Applications

doi: 10.3390/polym14030619

Figure Lengend Snippet: Summary of test methods.

Article Snippet: Surface unevenness , 3D-optical microscope (VR-3000, KEYENCE, Osaka, Japan) , ISO4287:1997 Surface unevenness-Definitions.

Techniques: Microscopy, Permeability

Journal: bioRxiv

Article Title: Cell cycle-dependent binding between Cyclin B1 and Cdk1 revealed by time-resolved Fluorescence Correlation Spectroscopy

doi: 10.1101/2022.02.22.481435

Figure Lengend Snippet: A) Flow cytometry profiles of Propidium iodide-stained RPE CCNB1-mEmerald +/+ cells: asynchronous and at 3, 6, 9 and 12 hours after release from Palbociclib-arrest. B) Plots of the FCS autocorrelation functions of Cyclin B1-mEmerald at the indicated time points after release from Palbociclib-arrest. Each dot represents the measurement from one cell. C) Graph showing time-resolved Cyclin B1-mEmerald concentration (left axis) and the fraction of Cyclin B1 in complex with Cdk1 (right axis) estimated from FCS measurements in the cytoplasm. D) Top panel: quantification of the protein levels measured from anti-Cyclin B1 and anti-Cdk1 immuno-blots (bottom panel, molecular mass indicated on the right) of either synchronized lysates (left) or Cyclin B1 immunoprecipitates (right). The ratio between Cdk1 and Cyclin B1 is plotted in red on the right axis. E) Quantification of Cyclin B1 fluorescence levels (normalised Raw Integrated Density, RID) over time measured by widefield fluorescence microscopy in unsynchronized RPE CCNB1-mEmerald +/+ cells. Orange dotted line indicates nuclear envelope breakdown. F) Quantification of FCS-derived measurements of the fraction of Cyclin B1 bound to Cdk1 over total Cyclin B1 levels, plotted against total Cyclin B1 concentration. In all graphs error bars indicate standard deviation. For all panels, N=2 independent experiments, time indicates hours after Palbociclib release.

Article Snippet: G2-synchronization was achieved through a 24-h treatment with 100 nM Palbociclib (Selleckchem) followed by 12 h release into normal medium, as described in ( , ).

Techniques: Flow Cytometry, Staining, Concentration Assay, Western Blot, Fluorescence, Microscopy, Derivative Assay, Standard Deviation

Journal: bioRxiv

Article Title: Cell cycle-dependent binding between Cyclin B1 and Cdk1 revealed by time-resolved Fluorescence Correlation Spectroscopy

doi: 10.1101/2022.02.22.481435

Figure Lengend Snippet: A) Graph representing the FCS autocorrelation functions of Cyclin B1-mEmerald at different the indicated time points after release from Palbociclib-arrest. Each dot represents the measurement from one cell. B) Graph showing time resolved Cyclin B1-mEmerald concentration (left axis) and the fraction of Cyclin B1 in complex with Cdk1 (right axis) estimated from FCS measurements in the nucleus. C) anti-Cyclin B1 and anti-Cdk1 immuno-blot of synchronized RPE CCNB1-mEmerald +/+ cells lysates after immunoprecipitation with anti-IgG antibody. D) Representative widefield images over time of a RPE CCNB1-mEmerald +/+ cells. Time is expressed at hh:mm. Scale bars correspond to 10 μm. For all panels, N=2 independent experiments, time indicates hours after Palbociclib release.

Article Snippet: G2-synchronization was achieved through a 24-h treatment with 100 nM Palbociclib (Selleckchem) followed by 12 h release into normal medium, as described in ( , ).

Techniques: Concentration Assay, Immunoprecipitation

Journal: bioRxiv

Article Title: Cell cycle-dependent binding between Cyclin B1 and Cdk1 revealed by time-resolved Fluorescence Correlation Spectroscopy

doi: 10.1101/2022.02.22.481435

Figure Lengend Snippet: A) Flow cytometry profiles of Propidium iodide-stained RPE CCNB1 - mEmerald +/+ Cdk1-mScarlet cells: asynchronous and at 3, 6, 9 and 12 hours after release from Palbociclib-arrest. B - D) Frequency histograms of the Log (K D ) values measured for Cyclin B1-mEmerald and Cdk1-mScarlet by FCCS (see ) at 6 (B), 9 (C), and 12 (D) hours after release from a Palbociclib-arrest. In all histograms, the dotted line represents the Gaussian fit. K D is indicated as mean ± SEM (Standard Error of the Mean). For all panels, N=2 independent experiments.

Article Snippet: G2-synchronization was achieved through a 24-h treatment with 100 nM Palbociclib (Selleckchem) followed by 12 h release into normal medium, as described in ( , ).

Techniques: Flow Cytometry, Staining

Journal: PLoS ONE

Article Title: Three Dimensional Collagen Scaffold Promotes Intrinsic Vascularisation for Tissue Engineering Applications

doi: 10.1371/journal.pone.0149799

Figure Lengend Snippet: (A) A 3D collagen scaffold measured to a diameter of 8 mm and a thickness of 2 mm. (B) Representative images captured by scanning electron microscopy on the surface (left) and through a cross section (right) of collagen scaffold.

Article Snippet: The growth of GFP-expressed human ASCs cultured in 3D collagen scaffolds or on collagen coated (0.5 mg/mL; Invitrogen) plate was assessed by using alamarBlue assay.

Techniques: Electron Microscopy

Journal: PLoS ONE

Article Title: Three Dimensional Collagen Scaffold Promotes Intrinsic Vascularisation for Tissue Engineering Applications

doi: 10.1371/journal.pone.0149799

Figure Lengend Snippet: (A) Representative images captured from 3D collagen scaffolds seeded with human ASCs for 24 hours: haematoxylin and eosin (H&E) staining and scanning electron microscopy (SEM). The white arrow demonstrates human ASCs attached to the collagen scaffold. (B) Representative images of GFP-expressed human ASCs cultured in collagen scaffolds for 7 and 21 days. Scale bar = 200 μm. (C) The proliferation rate of GFP-expressed human ASCs cultured in 3D collagen scaffolds and on 2D collagen-coated plates (n = 3). ** p <0.01 vs 2D culture by unpaired t-test.

Article Snippet: The growth of GFP-expressed human ASCs cultured in 3D collagen scaffolds or on collagen coated (0.5 mg/mL; Invitrogen) plate was assessed by using alamarBlue assay.

Techniques: Staining, Electron Microscopy, Cell Culture